Plant Tissue Culture, Development, and Biotechnology
Features
• Integrates traditional plant sciences with recent advances plant tissue culture, development and biotechnology
• Includes critical thinking questions, concept boxes introducing important ideas in the chapters, and procedure boxes giving precise instruction for experiments
• New chapters, concepts, and laboratory exercises provide information on germplasm preservation, plant growth regulators, proper microscope use with digital photography, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems
• Laboratory exercises provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed to perform them
• Special topics highlight copyrights, patents, legalities, and trade secrets in biotechnology
Summary
Addressing several specialized areas generically termed biotechnology, Plant Tissue Culture, Development and Biotechnology uses a broad range of species that are studied via laboratory exercises. Based on the editors’ previous textbooks Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises – Second Edition, the book provides detailed perspectives and hands-on training in this rather diverse field. It concentrates on plant development and its application in biotechnology, while maintaining as broad of a base as possible. Supporting laboratory exercises are provided.
Contents
Introduction
D. J. Gray and R. N. Trigiano
Supporting Methodologies and General Concepts
Getting Started with Tissue Culture—Media Preparation, Sterile Technique, and Laboratory Equipment
Caula A. Beyl
Nutrition of Cell and Organ Cultures
J. D. Caponetti and R. N. Trigiano
PGRs and Their Use in Micropropagation
Caula A. Beyl
Elements of In Vitro Research
Michael E. Compton
Proper Use of Microscopes
David T. Webb
Plant Histological Techniques
R. N. Trigiano, D. J. Gray, K. R. Malueg, K. A. Pickens, Z.-M. Cheng, and E. T. Graham
A Brief Introduction to Plant Anatomy and Morphology
R. N. Trigiano, Jennifer A. Franklin, and D. J. Gray
Seed Development and Germination
Feng Chen, Ruth C. Martin, Songquan Song, and Hiroyuki Nonogaki
Molecular Tools for Studying Plant Genetic Diversity
Timothy A. Rinehart, Xinwang Wang, R. N. Trigiano, Naomi R. Rowland, and Rene E. DeVries
Molecular Approaches to the Study of Plant Development
Albrecht G. von Arnim and Byung-Hoon Kim
Propagation and Development Concepts
Propagation by Shoot Culture
Michael Kane
Micropropagation of Syngonium by Shoot Culture
Michael Kane
Micropropagation and In Vitro Flowering of Rose
Michael Kane, Timothy Johnson, and Philip Kauth
Micropropagation of Potato by Node Culture and Microtuber Production
Michael Kane
Commercial Laboratory Production
G. R. L. Suttle
Detection and Elimination of Microbial Endophytes and Prevention of Contamination in Plant Tissue Culture
Alan C. Cassells
Culture Indexing for Bacterial and Fungal Contaminants
Michael Kane, Philip Kauth, and Timothy Johnson
Propagation from Nonmeristematic Tissues—Organogenesis
Robert L. Geneve
Developing a Molecular Understanding of In Vitro and In Planta Shoot Organogenesis
Ling Meng, Shibo Zhang, and Peggy G. Lemaux
Direct Shoot Organogenesis from Leaf Explants of Chrysanthemum and African Violets
R. N. Trigiano, L. M. Vito, M. T. Windham, Sarah Boggess, and Denita Hadziabdic
Propagation from Nonmeristematic Tissues—Nonzygotic Embryogenesis
D. J. Gray
Developmental and Molecular Aspects of Nonzygotic (Somatic) Embryogenesis
Xiyan Yang and Xianlong Zhang
Embryogenic Callus and Suspension Cultures from Leaves of Orchardgrass
D. J. Gray, R. N. Trigiano, and Bob V. Conger
Direct Nonzygotic Embryogenesis from Leaves and Flower Receptacles of Cineraria
R. N. Trigiano, M. C. Scott, and K. R. Malueg
Crop Improvement Techniques
Protoplasts—An Increasingly Valuable Tool in Plant Research
Jude W. Grosser and Ahmad A. Omar
Demonstration of Principles of Protoplast Isolation Using Chrysanthemum and Orchardgrass Leaves
R. N. Trigiano
Isolation, Culture, and Fusion of Tobacco and Potato Protoplasts
Richard E. Veilleux and Michael E. Compton
Haploid Cultures
Denita Hadziabdic, Phillip A. Wadl, and Sandra M. Reed
Production of Haploid Tobacco and Potato Plants Using Anther Culture
Phillip A. Wadl, Sandra M. Reed, and Denita Hadziabdic
Embryo Rescue
Tom Eeckhaut, Katrijn Van Laere, and Johan Van Huylenbroeck
Promoters and Gene Expression Regulation
Zhijian T. Li and D. J. Gray
Genetic Engineering Technologies
Zhijian T. Li, Sadanand A. Dhekney, and D. J. Gray
Transformation of Plant Meristems
Jean H. Gould
Genetic Transformation of Chysanthemum and Tobacco Using Agrobacterium tumefaciens
Margaret M. Young, Linas Padegimas, Nancy A. Reichert, and R. N. Trigiano
Genetic Transformation of Tobacco and Production of Transgenic Plants
Sadanand A. Dhekney, Zhijian T. Li, and D. J. Gray
Genetically Modified Controversies
H. A. Richards, L. C. Hudson, M. D. Halfhill, and C. N. Stewart, Jr.
Cryopreservation of Plant Cells, Tissues, and Organs
Barbara M. Reed, M. N. Normah, and Svetlana V. Kushnarenko
Cryopreservation of In Vitro Grown Shoot Tips
Svetlana V. Kushnarenko, Barbara M. Reed, and M. N. Normah
Cryopreservation of Orthodox and Recalcitrant Seed
M. N. Normah, W. K. Choo, Svetlana V. Kushnarenko, and Barbara M. Reed
Plant Biotechnology for the Production of Natural Products
Ara Kirakosyan, E. Mitchell Seymour, and Peter Kaufman
Pigment Production in Ajuga Cell Cultures
Mary Ann Lila and Randy B. Rogers
Variation in Tissue Culture
Margaret A. Norton and Robert M. Skirvin
The Business of Biotechnology
Biotechnology Entrepreneurship in the 21st Century
David W. Altman
Intellectual Property Protection for Plants
Chris Eisenschenk
Index
