Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition
Robert N. Trigiano, Dennis J. Gray
• Examines the history of plant tissue culture, basic methods, propagation techniques, crop improvement methods, and specialized applications
• Copiously illustrated with detailed drawings and black-and-white photographs
• Explores advanced topics on genetic transformation techniques, photographic methods and statistical analysis of data
• New procedure boxes allow reader quick step by step access to experimental protocols
Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research.
The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include "Micropropagation of Dieffenbachia," "Micropropagation and in vitro flowering of rose," "Propagation from nonmeristematic tissue-organogenesis," "Variation in culture" and "Tissue culture of ferns."
It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.
Table of Contents
(Note: Topic chapters appear below in bold upper and lower case letters; Laboratory chapters appear below in nonbold upper and lower case letters.)
PART I-INTRODUCTION
Introduction of plant tissue and cell culture
PART II-HISTORY OF PLANT TISSUE CULTURE
History of plant tissue and cell culture
PART III-SUPPORTING METHODOLOGIES
Getting started with tissue culture-Media preparation, sterile technique and laboratory equipment
Nutrition of callus cultures
Histological techniques
Microscopy and photography
Statistical analysis of plant tissue culture data
PART IV-PROPAGATION TECHNIQUES
Propagation from preexisting meristems
Micropropagation of Syngonium by shoot culture
Micropropagation of Dieffenbachia
Micropropagation of potato by node culture and microtuber production
Micropropagation of lilac
Micropropagation and in vitro flowering of rose
Propagation from nonmeristematic tissues- organogenesis
Direct shoot organogenesis from leaf explants of chrysanthemum
Shoot organogenesis of watermelon
Direct and indirect shoot organogenesis from leaves of Torenia fournieri
Shoot organogenesis from petunia leaves
Propagation from nonmeristematic tissues- Nonzygotic embryogenesis
Embryogenic callus and suspension cultures from leaves of orchardgrass
Somatic embryogenesis from seeds of melon
Somatic embryogenesis from mature peanut seed
Direct somatic embryogenesis from leaves and flower receptacles of cineraria
Somatic embryogenesis from immature seeds of yellow poplar
Introduction of somatic embryogenesis in conifers
PART V-CROP IMPROVEMENT TECHNIQUES
Use of protoplasts for plant improvement
Isolation, culture and fusion of tobacco and potato protoplasts
Isolation of protoplasts from leaves of chrysanthemum and orchardgrass
Haploid cultures
Production of haploid tobacco plants using another culture
Transformation
Transformation of tobacco and carrot using Agrobacterium tumefaciens and expression of the ί-glucuronidase (GUS) reporter gene
Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens
Construction and use of a simple gene gun for particle bombardment
Germplasm preservation
Vitrification as a method to cryopreserve shoot tips
Secondary product expression in vitro
Pigment production in Ajuga cell culture
In vitro plant pathology
PART VI-SPECIAL TOPICS
Variation in culture
Tissue culture of ferns
Commercial laboratory production
Indexing for plant pathogens
Culture indexing for bacterial and fungal contaminants
PART VII-APPENDIX I: LIST OF SUPPLIERS
PART VIII-INDEX
